• Catalog Number:

  E1A0548A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PKA α/β CAT around the phosphorylation site of threonine 197 (T-W-TP-L-C).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P17612/P22694

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000

• Other Names:

  cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; PRKACA ;PKACA ; cAMP-dependent protein kinase catalytic subunit beta; PKA C-beta; PRKACB

• Specificity Sensitivity:

  PKA α/β CAT (Phospho-Thr197) antibody detects endogenous levels of PKA α/β CAT only when phosphorylated at threonine 197.

• References:

  Ghislaine Garrel, Endocrinology, Jun 1997; 138: 2259. W Li, Learn. Mem., Jan 1996; 2: 320 - 333. Sharmin Schauble, J. Biol. Chem., May 2007; 282: 14952 - 14959. Yong G. Wang, J. Gen. Physiol., Jan 1998; 111: 113.

• All Sites:

  Human: Thr197; Mouse: Thr197; Rat: Thr197

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using PKA α/β CAT (Phospho-Thr197) antibody. Western blot analysis of extracts from mouse brain cells, using PKA α/β CAT (Phospho-Thr197) antibody.

P-peptide - + Immunofluorescence analysis of A549 cells, using PKA α/β CAT (Phospho-Thr197) antibody.