• Catalog Number:

  E1A0547A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PECAM-1 around the phosphorylation site of tyrosine 713 (TET-V-YP-S-EVRKAC).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P16284

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  CD31, CD31 antigen, EndoCAM, PEC1, PECA1, PECAM, PECAM1, Platelet endothelial cell adhesion molecule precursor

• Specificity Sensitivity:

  PECAM-1 (Phospho-Tyr713) antibody detects endogenous levels of PECAM-1 only when phosphorylated at tyrosine 713.

• References:

  Simmons D.L., J. Exp. Med. 171:2147-2152(1990). Famiglietti J., J. Cell Biol. 138:1425-1435(1997). Wang Y., Am. J. Physiol. 284:H1008-H1017(2003). ------------------------------------------------------------------------------------------------------------------------------------------

• All Sites:

  Human: Tyr713; Mouse: Tyr702

P-peptide - + Western blot analysis of extracts from Jurkat cells, using PECAM-1 (Phospho-Tyr713) antibody. Immunofluorescence analysis of HeLa cells, using PECAM-1 (Phospho-Tyr713) antibody.