• Catalog Number:

  E1A0543A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PDE4D around the phosphorylation site of serine 190/53 (R-E-SP-F-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q08499

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  DPDE3, EC 3.1.4.17, PDE43, PDE4D4, cAMP-specific 3', 5'-cyclic phosphodiesterase 4D

• Specificity Sensitivity:

  PDE4D (Phospho-Ser190/53) antibody detects endogenous levels of PDE4D only when phosphorylated at serine 190/53.

• References:

  Simon J. MacKenzie, J. Biol. Chem., May 2000; 275: 16609. CÉLINE MÉHATS, FASEB J, Oct 2003; 17: 1831. Guénaëlle Levallet, Biol Reprod, May 2007; 76: 794 - 803. Daniel Peter, J. Immunol., Apr 2007; 178: 4820 - 4831.

• All Sites:

  Human: Ser190; Mouse: Ser129; Rat: Ser185

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using PDE4D (Phospho-Ser190/53) antibody. Western blot analysis of extracts from K562 cells, treated with H2O2 (100uM, 30mins), using PDE4D (Phospho-Ser190/53) antibody.