• Catalog Number:

  E1A0535A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human P90RSK around the phosphorylation site of threonine 359/serine 363 (TP-P-K-D-SP).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q15418

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  90 kDa ribosomal protein S6 kinase 1, EC 2.7.11.1, KS6A1, MAPKAP-K1a, RPS6KA1, RSK-1, RSK1, Ribosomal S6 kinase 1, Ribosomal protein S6 kinase alpha 1, S6K-alpha 1, kinase p90RSK1, p90-RSK 1, pp90RSK1

• Specificity Sensitivity:

  p90 RSK (Phospho-Thr359+Ser363) antibody detects endogenous levels of P90RSK only when phosphorylated at threonine 359/serine 363.

• References:

  Eiichi Takahashi, J. Biol. Chem., Jul 1999; 274: 20206. Ye Zhang, J. Biol. Chem., May 2002; 277: 19042 - 19048. Ganesh R. Panta, Mol. Cell. Biol., Mar 2004; 24: 1823 - 1835. Yi Tan, J. Biol. Chem., Dec 1999; 274: 34859.

• All Sites:

  Human: Thr359/Ser363; Mouse: Ser352/Thr348; Rat: Ser363/Thr359

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using p90 RSK (Phospho-Thr359+Ser363) antibody. Western blot analysis of extracts from 293 cells, treated with PMA (125ng/ml, 30mins), using p90 RSK (Phospho-Thr359+Ser363) antibody.