• Catalog Number:

  E1A0534A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human P70S6K around the phosphorylation site of serine 418 (I-G-SP-P-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P23443

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  EC 2.7.11.1, KS6B1, P70-S6K, RPS6KB1, Ribosomal protein S6 kinase, Ribosomal protein S6 kinase, 70kDa, polypeptide 1, S6K, kinase p70S6K, p70-S6K

• Specificity Sensitivity:

  p70 S6 Kinase (Phospho-Ser418) antibody detects endogenous levels of P70S6K only when phosphorylated at serine 418.

• References:

  O. Jameel Shah, J. Biol. Chem., Jan 2002; 277: 2525 - 2533. Chuanshu Huang, Cancer Res., Oct 2002; 62: 5689 - 5697. Isabelle Briaud, Diabetes, Apr 2003; 52: 974.

• All Sites:

  Human: Ser418; Mouse: Ser441; Rat: Ser418

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase (Phospho-Ser418) antibody. Western blot analysis of extracts from HT-29 cells, treated with serum (20%, 15mins), using p70 S6 Kinase (Phospho-Ser418) antibody.

P-peptide - + Immunofluorescence analysis of COS7 cells, using p70 S6 Kinase (Phospho-Ser418) antibody.