• Catalog Number:

  E1A0531A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human P70S6K around the phosphorylation site of threonine 229 (T-H-TP-F-C).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P23443

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  EC 2.7.11.1, KS6B1, P70-S6K, RPS6KB1, Ribosomal protein S6 kinase, Ribosomal protein S6 kinase, 70kDa, polypeptide 1, S6K, kinase p70S6K, p70-S6K

• Specificity Sensitivity:

  p70 S6 Kinase (Phospho-Thr229) antibody detects endogenous levels of P70S6K only when phosphorylated at threonine 229.

• References:

  O. Jameel Shah, J. Biol. Chem., Jan 2002; 277: 2525 - 2533. Chuanshu Huang, Cancer Res., Oct 2002; 62: 5689 - 5697. O. Jameel Shah, Am J Physiol Endocrinol Metab, Jul 2000; 279: E74 - E82. Isabelle Briaud, Diabetes, Apr 2003; 52: 974.

• All Sites:

  Human: Thr229; Mouse: Thr252; Rat: Thr229

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using p70 S6 Kinase (Phospho-Thr229) antibody. Western blot analysis of extracts from Jurkat cells, using p70 S6 Kinase (Phospho-Thr229) antibody.