• Catalog Number:

  E1A0530A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p53 around the phosphorylation site of serine 392 (E-G-P-D-SP).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P04637

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  Antigen NY-CO-13, Cellular tumor antigen p53, Phosphoprotein p53, TP53, Tumor suppressor p53

• Specificity Sensitivity:

  p53 (Phospho-Ser392) antibody detects endogenous levels of p53 only when phosphorylated at serine 392.

• References:

  Lloyd M. Alderson, Cancer Res., Mar 1995; 55: 999 - 1001. WE Mercer, PNAS, Aug 1990; 87: 6166. Timothy J. Underwood, AACR Meeting Abstracts, Apr 2006; 2006: 1167. Jean-Christophe Bourdon, Genes & Dev., Sep 2005; 19: 2122 - 2137.

• All Sites:

  Human: Ser392

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using p53 (Phospho-Ser392) antibody. Western blot analysis of extracts from 293 cells treated with Etoposide (25uM, 60mins) and COS7 cells treated with EGF( 200ng/ml, 30mins), using p53 (Phospho-Ser392) antibody.

P-peptide - + Immunofluorescence analysis of HeLa cells, using p53 (Phospho-Ser392) antibody.