• Catalog Number:

  E1A0528A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p300 around the phosphorylation site of serine 89 (S-G-SP-S-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q09472

• Applications:

  IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  E1A-associated protein p300, EC 2.3.1.48, EP300, P300 transcription coactivator homolog

• Specificity Sensitivity:

  p300 (Phospho-Ser89) antibody detects endogenous levels of p300 only when phosphorylated at serine 89.

• References:

  Eckner R., Genes Dev. 8:869-884(1994). Dunham I., Nature 402:489-495(1999). Chaffanet M., Genes Chromosomes Cancer 28:138-144(2000).

• All Sites:

  Human: Ser89; Mouse: Ser89; Rat: Ser89

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue using p300 (Phospho-Ser89) antibody.