• Catalog Number:

  E1A0527A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human n-NOS around the phosphorylation site of serine 847 (F-N-SP-V-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P29476

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  BNOS, Constitutive NOS, EC 1.14.13.39, N-NOS, NC-NOS, NOS, type I, NOS1, Neuronal NOS, Nitric-oxide synthase, brain, bNOS

• Specificity Sensitivity:

  n-NOS (Phospho-Ser852) antibody detects endogenous levels of n-NOS only when phosphorylated at serine 847.

• References:

  George D. Webb, J. Thorac. Cardiovasc. Surg., Nov 2006; 132: 1131 - 1136. Montserrat García–Vitoria, J. Histochem. Cytochem., Aug 2000; 48: 1111. Peter J. Reiser, J Appl Physiol, Apr 1997; 82: 1250. VL Dawson, J. Neurosci., Apr 1996; 16: 2479.

• All Sites:

  Human: Ser852; Mouse: Ser847; Rat: Ser847

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using n-NOS (Phospho-Ser852) antibody. Western blot analysis of extracts from A549 cells, using n-NOS (Phospho-Ser852) antibody.

P-peptide - + Immunofluorescence analysis of HeLa cells, using n-NOS (Phospho-Ser852) antibody.