Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human NMDAR2B around the phosphorylation site of tyrosine 1474 (H-V-YP-E-K).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
NMDAR2B (Phospho-Tyr1474) antibody detects endogenous levels of NMDAR2B only when phosphorylated at tyrosine 1474.
References:
Carola G. Schipke, FASEB J, Mar 2001; 10.1096/fj.00-0439fje.
Myoung Sook Kim, Cancer Res., Apr 2006; 66: 3409 - 3418.
MA Varney, J. Pharmacol. Exp. Ther., Oct 1996; 279: 367.
Nobuhiko Kojima, Learn. Mem., Nov 1998; 5: 429 - 445.
All Sites:
Human: Tyr1474; Mouse: Tyr1472; Rat: Tyr1472
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human brain tissue using NMDAR2B (Phospho-Tyr1474) antibody.