• Catalog Number:

  E1A0525A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human NF-κB p65 around the phosphorylation site of threonine 505 (L-V-TP-G-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 100%);Rat (Identities = 92%, Positives = 100%)

• SwissProtNo:

  Q04206

• Applications:

  IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  NFKB3, Nuclear factor NF-kappa-B p65 subunit, RELA, TF65, Transcription factor p65, p65

• Specificity Sensitivity:

  NF-κB p65 (Phospho-Thr505) antibody detects endogenous levels of NF-κB p65 only when phosphorylated at threonine 505.

• References:

  Ruben S.M., Science 251:1490-1493(1991). Deloukas P., Hum. Mol. Genet. 2:1895-1900(1993). Lyle R., Gene 138:265-266(1994).

• All Sites:

  Human: Thr505; Mouse: Thr504; Rat: Thr505

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using NF-κB p65 (Phospho-Thr505) antibody.