• Catalog Number:

  E1A0522A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human NFAT4 around the phosphorylation site of serine 165 (S-P-SP-P-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q12968

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  NF-AT4, NF-ATc3, NFAC3, NFATC3, NFATx, NFC3, Nuclear factor of activated T-cells, cytoplasmic 3, T cell transcription factor NFAT4

• Specificity Sensitivity:

  NFAT4 (Phospho-Ser165) antibody detects endogenous levels of NFAT4 only when phosphorylated at serine 165.

• References:

  Chi-Wing Chow, Science, Nov 1997; 278: 1638. Kate Manley, J. Virol., Dec 2006; 80: 12079 - 12085. Andra S. Stevenson, J. Biol. Chem., Apr 2001; 276: 15018 - 15024. Jingtao Chen, J. Immunol., Mar 2003; 170: 3109.

• All Sites:

  Human: Ser165; Mouse: Ser165

P-peptide - + Western blot analysis of extracts from HeLa cells, treated with Ca+ (40nM, 30mins), using NFAT4 (Phospho-Ser165) antibody. Immunofluorescence analysis of HeLa cells, using NFAT4 (Phospho-Ser165) antibody.