• Catalog Number:

  E1A0521A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human NFAT3 around the phosphorylation site of serine 676 (K-R-SP-P-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q14934

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  NF-AT3, NF-ATc4, NFAC4, NFATC4, NFC4, Nuclear factor of activated T-cells, cytoplasmic 4, T cell transcription factor NFAT3, Transcription complex subunit NF-ATc4

• Specificity Sensitivity:

  NFAT3 (Phospho-Ser676) antibody detects endogenous levels of NFAT3 only when phosphorylated at serine 676.

• References:

  Hoey T., Immunity 2:461-472(1995). Crabtree G.R.; Cell 96:611-614(1999). Olsen J.V., Cell 127:635-648(2006).

• All Sites:

  Human: Ser676; Mouse: Ser676

Western blot analysis of extracts from HepG2 cells, treated with Ca2+ (40μM, 30mins), using NFAT3 (Phospho-Ser676) antibody.