• Catalog Number:

  E1A0518A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MYPT1 around the phosphorylation site of threonine 853 (R-S-TP-G-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O14974

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  MBS, Myosin phosphatase target subunit 1, PPP1R12A

• Specificity Sensitivity:

  MYPT1 (Phospho-Thr853) antibody detects endogenous levels of MYPT1 only when phosphorylated at threonine 853.

• References:

  Kevin B. Atkins, Am J Physiol Heart Circ Physiol, Jul 2007; 293: H402 - H408. Allison M. Given, Am J Physiol Cell Physiol, Jan 2007; 292: C432 - C439. Haiying Zhang, Circ. Res., Mar 2007; 100: 730 - 737. Mikiharu Yoshida, J. Biochem. (Tokyo), Feb 1988; 103: 380 - 385.

• All Sites:

  Human: Thr853; Mouse: Thr852; Rat: Thr855

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using MYPT1 (Phospho-Thr853) antibody. Western blot analysis of extracts from NIH/3T3 cells, using MYPT1 (Phospho-Thr853) antibody.