• Catalog Number:

  E1A0516A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MYOD around the phosphorylation site of serine 200 (A-S-SP-P-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P15172

• Applications:

  WB: 1:500~1:1000 ELISA: 1:5000

• Other Names:

  MYF3, MYOD, MYOD1, Myoblast determination protein 1, Myogenic factor MYF-3

• Specificity Sensitivity:

  MYOD (Phospho-Ser200) antibody detects endogenous levels of MYOD only when phosphorylated at serine 200.

• References:

  Pearson-White S.H.; Nucleic Acids Res. 19:1148-1148(1991). Braun T., EMBO J. 8:3617-3625(1989). Breitschopf K., EMBO J. 17:5964-5973(1998).

• All Sites:

  Human: Ser200; Mouse: Ser200; Rat: Ser200

Western blot analysis of extracts from Jurkat cells, treated with Ca+ (40nM, 30mins), using MYOD (Phospho-Ser200) antibody.