• Catalog Number:

  E1A0515A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MYB around the phosphorylation site of serine 12 (Y-S-SP-D-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P10242

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  C-myb; Myb proto-oncogene protein

• Specificity Sensitivity:

  MYB (Phospho-Ser12) antibody detects endogenous levels of MYB only when phosphorylated at serine 12.

• References:

  Majello B., Proc. Natl. Acad. Sci. U.S.A. 83:9636-9640(1986). Westin E.H., Oncogene 5:1117-1124(1990). Dasgupta P., Oncogene 4:1419-1423(1989).

• All Sites:

  Human: Ser12; Mouse: Ser12

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human heart tissue using MYB (Phospho-Ser12) antibody. Western blot analysis of extracts from HeLa cells, treated with Hu (2nM, 24hours), using MYB (Phospho-Ser12) antibody.