• Catalog Number:

  E1A0514A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MUNC-18a around the phosphorylation site of serine 313 (S-S-SP-K-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P61764

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  N- Sec1, STB1, STXB1, STXBP1, Syntaxin binding protein 1, UNC18A, Unc-18 homolog, Unc-18-1, Unc-18A, p67, rbSec1

• Specificity Sensitivity:

  MUNC-18a (Phospho-Ser313) antibody detects endogenous levels of MUNC-18a only when phosphorylated at serine 313.

• References:

  Gengyo-Ando K., J. Neurosci. 16:6695-6702(1996). Swanson D.A., Genomics 48:373-376(1998). The MGC Project Team; Genome Res. 14:2121-2127(2004).

• All Sites:

  Human: Ser313; Mouse: Ser313; Rat: Ser313

Western blot analysis of extracts from COS cells, treated with PMA (125ng/ml, 30mins), using MUNC-18a (Phospho-Ser313) antibody.