• Catalog Number:

  E1A0512A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MITF around the phosphorylation site of serine 73 (P-N-SP-P-M).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  O75030

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  MITF M1, Microphthalmia-associated transcription factor

• Specificity Sensitivity:

  MITF (Phospho-Ser180/73) antibody detects endogenous levels of MITF only when phosphorylated at serine 73.

• References:

  Jón H. Hallsson, Genetics, May 2004; 167: 233 - 241. Eiichi Morii, Blood, Apr 2001; 97: 2038 - 2044. Eiichi Morii, Blood, Sep 2004; 104: 1656 - 1661. Fatima Lekmine, AACR Meeting Abstracts, Apr 2005; 2005: 394.

• All Sites:

  Human: Ser180; Mouse: Ser180

Western blot analysis of extracts from COS7 cells, using MITF (Phospho-Ser180/73) antibody.