Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Met around the phosphorylation site of tyrosine1356 (A-T-YP-V-N).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q09YK0
Applications:
WB: 1:500~1:1000 ELISA: 1:10000
Other Names:
EC 2.7.10.1, HGF receptor, HGF-SF receptor, Hepatocyte growth factor receptor precursor, Met proto- oncogene tyrosine kinase, c-met, kinase Met
Specificity Sensitivity:
Met (Phospho-Tyr1356) antibody detects endogenous levels of Met only when phosphorylated at tyrosine1356.
References:
Antonellis A., Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
All Sites:
Human: Tyr1356; Mouse: Tyr1354; Rat: Tyr1357
Western blot analysis of extracts from 3T3 cells, using Met (Phospho-Tyr1356) antibody.