• Catalog Number:

  E1A0508A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MDM2 around the phosphorylation site of serine 166 (A-I-SP-E-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q00987

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  Double minute 2 protein, EC 6.3.2.-, Oncoprotein Mdm2, Ubiquitin-protein ligase E3 Mdm2, p53-binding protein Mdm2

• Specificity Sensitivity:

  MDM2 (Phospho-Ser166) antibody detects endogenous levels of MDM2 only when phosphorylated at serine 166.

• References:

  M Zhou, Blood, Mar 1995; 85: 1608 - 1614. Susan M. Mendrysa, Mol. Cell. Biol., Mar 2000; 20: 2023 - 2030. M. Saeed Sheikh, Cancer Res., Jul 1993; 53: 3226 - 3228. Jordan S. Fridman, Cancer Res., Sep 2003; 63: 5703 - 5706.

• All Sites:

  Human: Ser166; Mouse: Ser163

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MDM2 (Phospho-Ser166) antibody. Western blot analysis of extracts from COS7 cells, using MDM2 (Phospho-Ser166) antibody.

P-peptide - + Immunofluorescence analysis of A549 cells, using MDM2 (Phospho-Ser166) antibody.