• Catalog Number:

  E1A0505A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human LCK around the phosphorylation site of tyrosine 505 (G-Q-YP-Q-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P06239

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:60000

• Other Names:

  EC 2.7.10.2, LCK, LSK, LSK-T, P56-LCK, T cell-specific protein-tyrosine kinase, kinase Lck

• Specificity Sensitivity:

  Lck (Phospho-Tyr505) antibody detects endogenous levels of LCK only when phosphorylated at tyrosine 505.

• References:

  K M Tchou-Wong, Mol. Cell. Biol., Jan 1992; 12: 394 - 401. Marit Inngjerdingen, Blood, May 2002; 99: 4318 - 4325. K Lin, Int. Immunol., Feb 1997; 9: 291 - 306. Kavita Tewari, PNAS, Oct 2006; 103: 16388 - 16393.

• All Sites:

  Human: Tyr505; Mouse: Tyr505

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Lck (Phospho-Tyr505) antibody. Western blot analysis of extracts from Jurkat cells, using Lck (Phospho-Tyr505) antibody.