• Catalog Number:

  E1A0502A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human KIT around the phosphorylation site of tyrosine 703 (A-L-YP-K-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P10721

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  CD117, EC 2.7.10.1, Mast/stem cell growth factor receptor precursor, SCFR, SL, c-kit, kinase Kit

• Specificity Sensitivity:

  KIT (Phospho-Tyr703) antibody detects endogenous levels of KIT only when phosphorylated at tyrosine 703.

• References:

  Liu T., J. Proteome Res. 4:2070-2080(2005). Syrris P., m. J. Med. Genet. 95:79-81(2000).. Bignell G., Genes Chromosomes Cancer 45:42-46(2006).

• All Sites:

  Human: Tyr703; Mouse: Tyr702

Western blot analysis of extracts from HepG2 cells, treated with EGF (200ng/ml, 30mins), using KIT (Phospho-Tyr703) antibody.