• Catalog Number:

  E1A0501A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human KIT around the phosphorylation site of tyrosine 936 (H-I-YP-S-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P10721

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  c-kit; CD117; EC 2.7.10.1; kinase Kit; Mast/stem cell growth factor receptor precursor; SCFR; SL; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

• Specificity Sensitivity:

  KIT (Phospho-Tyr936) antibody detects endogenous levels of KIT only when phosphorylated at tyrosine 936.

• References:

  Yarden Y., EMBO J. 6:3341-3351(1987). Giebel L.B., Oncogene 7:2207-2217(1992). Andre C., Genomics 39:216-226(1997).

• All Sites:

  Human: Tyr936; Mouse: Tyr934

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human heart tissue using KIT (Phospho-Tyr936) antibody. Western blot analysis of extracts from HepG2 cells, treated with EGF (200ng/ml, 30mins), using KIT (Phospho-Tyr936) antibody.