• Catalog Number:

  E1A0500A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human JNK1/2/3 around the phosphorylation site of threonine183/tyrosine 185 (M-M-TP-P-YP-V-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P45983/P45984/P53779

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

  EC 2.7.11.24, JNK-46, JNK1-alpha-2, MAPK8, MK08, Mitogen-activated protein kinase 8, PRKM8, SAPK gamma, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, kinase JNK1, p54 gamma

• Specificity Sensitivity:

  JNK1/2/3 (Phospho-Thr183+Tyr185) antibody detects endogenous levels of JNK1/2/3 only when phosphorylated at threonine183/tyrosine 185.

• References:

  Robert A. Kaiser, J. Biol. Chem., Sep 2005; 280: 32602 - 32608. Eleanor T. Coffey, J. Neurosci., Jun 2002; 22: 4335. Nanyue Chen, Cancer Res., Mar 2002; 62: 1300 - 1304. Nan-Shan Chang, J. Biol. Chem., Mar 2003; 278: 9195 - 9202.

• All Sites:

  Human: Thr183/Tyr185; Mouse: Thr183/Tyr185; Rat: Thr183/Tyr185

Western blot analysis of extracts from 293 cells, treated with UV (5mins), using JNK1/2/3 (Phospho-Thr183+Tyr185) antibody.