• Catalog Number:

  E1A0499A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human JAK2 around the phosphorylation site of tyrosine 750 (G-D-YP-G-Q).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O60674

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  EC 2.7.10.2, JAK-2, JAK2, Janus kinase 2, kinase Jak2

• Specificity Sensitivity:

  JAK2 (Phospho-Tyr570) antibody detects endogenous levels of JAK2 only when phosphorylated at tyrosine 750.

• References:

  Saltzman A., Biochem. Biophys. Res. Commun. 246:627-633(1998). Dalal I., Blood 91:844-851(1998). Peeters P., Blood 90:2535-2540(1997).

• All Sites:

  Human: Tyr750; Mouse: Tyr750; Rat: Tyr750

Western blot analysis of extracts from 293 cells, treated with etoposide (25uM, 24hours), using JAK2 (Phospho-Tyr570) antibody.