• Catalog Number:

  E1A0497A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 323 (P-G-SP-F-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P35568

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000

• Other Names:

  IRS-1, IRS1, Insulin receptor substrate-1

• Specificity Sensitivity:

  IRS-1 (Phospho-Ser323) antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 323.

• References:

  Joëlle Dupont, Am J Physiol Endocrinol Metab, Feb 1998; 274: E309 - E316. DG Baskin, Endocrinology, Apr 1994; 134: 1952. Zhefu Ma,. Mol. Cell. Biol., Dec 2006; 26: 9338 - 9351. Alexandros Tzatsos, J. Biol. Chem., Jun 2007; 282: 18069 - 18082.

• All Sites:

  Human: Ser323; Mouse: Ser318; Rat: Ser318

Western blot analysis of extracts from COS7 cells, treated with Serum (20%, 15mins), using IRS-1 (Phospho-Ser323) antibody.