• Catalog Number:

  E1A0496A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IRF-3 around the phosphorylation site of serine 385 (G-A-SP-S-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q14653

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000

• Other Names:

  Interferon regulatory factor 3; IRF3

• Specificity Sensitivity:

  IRF-3 (Phospho-Ser385) antibody detects endogenous levels of IRF-3 only when phosphorylated at serine 385.

• References:

  Au W.W.-C., Proc. Natl. Acad. Sci. U.S.A. 92:11657-11661(1995). The MGC Project Team; Genome Res. 14:2121-2127(2004). Bellingham J., Ann. Hum. Genet. 62:231-234(1998).

• All Sites:

  Human: Ser385; Mouse: Ser387; Rat: Ser380

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using IRF-3 (Phospho-Ser385) antibody. Western blot analysis of extracts from HT-29 cells, treated with insulin (0.01U/ml, 15mins), using IRF-3 (Phospho-Ser385) antibody.

P-peptide - + Immunofluorescence analysis of HeLa cells, using IRF-3 (Phospho-Ser385) antibody.