• Catalog Number:

  E1A0493A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IR around the phosphorylation site of tyrosine 1355 (R-S-YP-E-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P06213

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  CD220 antigen, EC 2.7.10.1, IR, insulin receptor, kinase InsR

• Specificity Sensitivity:

  IR (Phospho-Tyr1355) antibody detects endogenous levels of IR only when phosphorylated at tyrosine 1355.

• References:

  Laura Sciacca, Endocrinology, Jun 2003; 144: 2650. Mark Westcott, Asia Pacific Journal of Human Resources, Aug 2003; 41: 172 - 189. Adam Denley, Mol. Endocrinol., Oct 2004; 18: 2502 - 2512. Rita Slaaby, J. Biol. Chem., Sep 2006; 281: 25869 - 25874.

• All Sites:

  Human: Tyr1355; Mouse: Tyr1345; Rat: Tyr1356

Western blot analysis of extracts from 293 cells, treated with Heat shock, using IR (Phospho-Tyr1355) antibody.