• Catalog Number:

  E1A0490A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IKK-β around the phosphorylation site of tyrosine 199 (Q-K-YP-T-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O14920

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  EC 2.7.11.10, I-kappa-B kinase 2, I-kappa-B-kinase beta, IKK-B, IKK-beta, IKK2, IKKB, IkBKB, NFKBIKB, Nuclear factor NF-kappa-B inhibitor kinase beta, kinase IKK-beta

• Specificity Sensitivity:

  IKK-β (Phospho-Tyr199) antibody detects endogenous levels of IKK-β only when phosphorylated at tyrosine 199.

• References:

  Zhi-Wei Li, J. Immunol., May 2003; 170: 4630. Wen-Hui Hu, J. Biol. Chem., Aug 2005; 280: 29233 - 29241. S. Escotte, Eur. Respir. J., Apr 2003; 21: 574 - 581. Dirk Haller, J. Biol. Chem., Oct 2002; 277: 38168 - 38178.

• All Sites:

  Human: Tyr199; Mouse: Tyr199; Rat: Tyr199

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using IKK-β (Phospho-Tyr199) antibody. Western blot analysis of extracts from HeLa cells, treated with TNF-a (20ng/ml) and Calyculin (50nM, 5mins), using IKK-β (Phospho-Tyr199) antibody.