• Catalog Number:

  E1A0488A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HSP20 around the phosphorylation site of serine 16 (R-A-SP-A-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O14558

• Applications:

  IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  HSPB6, Heat-shock 20 kDa like-protein p20

• Specificity Sensitivity:

  HSP20 (Phospho-Ser16) antibody detects endogenous levels of HSP20 only when phosphorylated at serine 16.

• References:

  Guo-Chang Fan, Circ. Res., Jun 2004; 94: 1474 - 1482. Junzo Norimine, Infect. Immun., Feb 2004; 72: 1096 - 1106. Marco Ventura, Appl. Envir. Microbiol., Jul 2007; 73: 4695 - 4703. Charles R. Flynn, FASEB J, May 2003; 10.1096/fj.02-1028fje.

• All Sites:

  Human: Ser16; Mouse: Ser16; Rat: Ser16

P-peptide - +

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using HSP20 (Phospho-Ser16) antibody. Immunofluorescence analysis of HeLa cells, using HSP20 (Phospho-Ser16) antibody.