• Catalog Number:

  E1A0486A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human GRK2 around the phosphorylation site of serine 29 (R-A-SP-K-K).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P25098

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  ADRBK1, ARBK1, BARK, BARK1, Beta-ARK-1, Beta-adrenergic receptor kinase 1, EC 2.7.11.15, G protein receptor kinase 2, G- protein coupled receptor kinase 2, kinase GRK2

• Specificity Sensitivity:

  GRK2 (Phospho-Ser29) antibody detects endogenous levels of GRK2 only when phosphorylated at serine 29.

• References:

  Aarti N. Desai, J. Pharmacol. Exp. Ther., Jun 2006; 317: 1027 - 1035. John A. Gray, Mol. Pharmacol., Nov 2001; 60: 1020. Anne Vroon, J. Leukoc. Biol., May 2004; 75: 901 - 909. R. J. Hughes, Am J Physiol Cell Physiol, Mar 1996; 270: C885 - C891.

• All Sites:

  Human: Ser29; Mouse: Ser29; Rat: Ser29

Western blot analysis of extracts from Jurkat cells, treated with EGF (200ng/ml, 30mins), using GRK2 (Phospho-Ser29) antibody.