• Catalog Number:

  E1A0485A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human FOXO1A/3A around the phosphorylation site of serine 322 and serine 325 (N-A-SP-T-I-SP-G-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q12778

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  FKHR, FOXO1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma

• Specificity Sensitivity:

  FOXO1A/3A (Phospho-Ser322+Ser325) antibody detects endogenous levels of FOXO1A/3A only when phosphorylated at serine 322 and serine 325.

• References:

  Andrew Sunters, J. Biol. Chem., Dec 2003; 278: 49795 - 49805. Philippe R.J. Bois, J. Cell Biol., Sep 2005; 170: 903 - 912. Walbert J. Bakker, J. Cell Biol., Jan 2004; 164: 175. Hien Tran, Science, Apr 2002; 296: 530.

• All Sites:

  Human: Ser322+Ser325; Mouse: Ser319+Ser322; Rat: Ser316+Ser319

Western blot analysis of extracts from NIH/3T3cells, treated with Wortmannin (40nM, 24hours), using FOXO1A/3A (Phospho-Ser322+Ser325) antibody.