• Catalog Number:

  E1A0483A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human FOXO1/3/4-pan around the phosphorylation site of threonine 24/32 (S-C-TP-W-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q12778/O43524/P98177

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  FKHR, FOXO1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, AF6q21 protein, FKHR2, FKHRL1, FOXO3, Forkhead box O3, Forkhead box protein O3A, Forkhead in rhabdomyosarcoma-like 1, AFX, AFX1, Afxh, Forkhead box protein O4, MLLT7, Putative forkhead domain AFX1, putative fork head domain AFX1

• Specificity Sensitivity:

  FOXO1/3/4-pan (Phospho-Thr24/32) antibody detects endogenous levels of FOXO1/3/4-pan only when phosphorylated at threonine 24/32.

• References:

  Robert J. Southgate, J. Biol. Chem., Jul 2007; 282: 21176 - 21186. Susumu Kodama, Mol. Cell. Biol., Sep 2004; 24: 7931 - 7940. Dragan Marinkovic, J. Clin. Invest., Aug 2007; 117: 2133 - 2144. Marie-Liesse Asselin-Labat, Blood, Jul 2004; 104: 215 - 223.

• All Sites:

  Human: Thr24/32; Mouse: Thr24/32; Rat: Thr24/32

Western blot analysis of extracts from 293 cells, treated with Serum (20%, 15mins), using FOXO1/3/4-pan (Phospho-Thr24/32) antibody.