• Catalog Number:

  E1A0482A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human FLT3 around the phosphorylation site of tyrosine 599 (Y-E-YP-D-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P36888

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  CD135, EC 2.7.10.1, FL cytokine receptor precursor, FLK-2, FLT-3, FLT3, Fetal liver kinase 2, STK-1, STK1, Stem cell tyrosine kinase 1, flk-2, kinase Flt3

• Specificity Sensitivity:

  FLT3 (Phospho-Tyr599) antibody detects endogenous levels of FLT3 only when phosphorylated at tyrosine 599.

• References:

  Small D., Proc. Natl. Acad. Sci. U.S.A. 91:459-463(1994). Rosnet O., Blood 82:1110-1119(1993). Greenman C., Nature 446:153-158(2007).

• All Sites:

  Human: Tyr599; Mouse: Tyr600

Western blot analysis of extracts from COS7 cells, treated with EGF (200ng/ml, 30mins), using FLT3 (Phospho-Tyr599) antibody.