• Catalog Number:

  E1A0481A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human FGFR1 around the phosphorylation site of tyrosine 654 (D-Y-YP-K-K).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P11362

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  EC 2.7.10.1, FGFBR, FGFR-1, FGR1, FLG, FLT2, Fms-like tyrosine kinase-2, MFR, bFGF-R, c-fgr, kinase FGFR1

• Specificity Sensitivity:

  FGFR1 (Phospho-Tyr654) antibody detects endogenous levels of FGFR1 only when phosphorylated at tyrosine 654.

• References:

  Chitta S. Kasyapa, Blood, May 2006; 107: 3693 - 3699. Benoit Rousseau, Cancer Res., Apr 2004; 64: 2490 - 2495. Takayuki Nakayama, Blood, Feb 2007; 109: 1363 - 1372.

• All Sites:

  Human: Tyr654; Mouse: Tyr654; Rat: Tyr561

P-peptide - + Western blot analysis of extracts from 293 cells, treated with Insulin (0.01U/ml, 15mins), using FGFR1 (Phospho-Tyr654) antibody. Immunofluorescence analysis of COS7cells, using FGFR1 (Phospho-Tyr654) antibody.