• Catalog Number:

  E1A0479A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human FAK around the phosphorylation site of tyrosine 407 (D-T-YP-T-M).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q05397

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  EC 2.7.10.2, FADK 1, FAK1, Focal adhesion kinase 1, PTK2, Protein- tyrosine kinase 2, pp125FAK

• Specificity Sensitivity:

  FAK (Phospho-Tyr407) antibody detects endogenous levels of FAK only when phosphorylated at tyrosine 407.

• References:

  Graham Jones. Cancer Res., Aug 2001; 61: 5688 - 5691. Chuanhai Zheng. J. Biol. Chem., Jan 1998; 273: 2384. Ian S. Hitchcock. Blood (ASH Annual Meeting Abstracts), Nov 2006; 108: 354.

• All Sites:

  Human: Tyr407; Mouse: Tyr438; Rat: Tyr407

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using FAK (Phospho-Tyr407) antibody. Western blot analysis of extracts from COS-7 cells, treated with EGF (200ng/ml, 30mins), using FAK (Phospho-Tyr407) antibody.