• Catalog Number:

  E1A0477A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ERF around the phosphorylation site of threonine 526 (P-L-TP-P-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P50548

• Applications:

  IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  ETS-domain transcription factor ERF, Ets2 repressor factor

• Specificity Sensitivity:

  ERF (Phospho-Thr526) antibody detects endogenous levels of ERF only when phosphorylated at threonine 526.

• References:

  Chara Papadaki, Mol. Cell. Biol., Jul 2007; 27: 5201 - 5213. Lionel Le Gallic, Mol. Cell. Biol., Feb 2004; 24: 1206 - 1218. Lisa A. McPherson, PNAS, Apr 1997; 94: 4342. Luis Oñate-Sánchez, Plant Physiology, Jan 2007; 143: 400 - 409.

• All Sites:

  Human: Thr526; Mouse: Thr529

P-peptide - +

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using ERF (Phospho-Thr526) antibody. Immunofluorescence analysis of HeLa cells, using ERF (Phospho-Thr526) antibody.