• Catalog Number:

  E1A0476A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ER-α around the phosphorylation site of tyrosine 537 (P-L-YP-D-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P03372

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  ER, ESR, ESR1, ESTR, ESTRA, Estradiol receptor, Estrogen receptor, NR3A1

• Specificity Sensitivity:

  Estrogen Receptor-α (Phospho-Tyr537) antibody detects endogenous levels of ER-α only when phosphorylated at tyrosine 537.

• References:

  Sehime Temel, Endocrinology, Oct 2002; 143: 3974. Xin-Tian Zhang, AACR Meeting Abstracts, Apr 2005; 2005: 302. Suwit J. Somponpun, Am J Physiol Regulatory Integrative Comp Physiol, Sep 2004; 287: R661 - R669. Marcello Maggiolini, Cancer Res., Oct 1999; 59: 4864 - 4869.

• All Sites:

  Human: Tyr537; Mouse: Tyr451

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Estrogen Receptor-α (Phospho-Tyr537) antibody. Western blot analysis of extracts from 293 cells, using Estrogen Receptor-α (Phospho-Tyr537) antibody.