• Catalog Number:

  E1A0475A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human EGFR around the phosphorylation site of tyrosine 1061 (D-E-YP-L-I).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P00533

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  EC 2.7.10.1, ERBB1, Epidermal growth factor receptor precursor, Receptor protein-tyrosine kinase ErbB-1, kinase EGFR

• Specificity Sensitivity:

  EGFR (Phospho-Tyr1016) antibody detects endogenous levels of EGFR only when phosphorylated at tyrosine 1061.

• References:

  S Leibl. J. Clin. Pathol., Jul 2005; 58: 700 - 704. Dhara N. Amin. Cancer Res., Feb 2006; 66: 2173 - 2180. B. Corkery. ASCO Meeting Abstracts, Jun 2007; 25: 14071. James G. Christensen. Clin. Cancer Res., Dec 2001; 7: 4230.

• All Sites:

  Human: Tyr1016; Mouse: Tyr1018; Rat: Tyr1017

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using EGFR (Phospho-Tyr1016) antibody. Western blot analysis of extracts from HuvEc cells, treated with Serum (20%, 30mins), using EGFR (Phospho-Tyr1016) antibody.

P-peptide - + Immunofluorescence analysis of HeLa cells, using EGFR (Phospho-Tyr1016) antibody.