• Catalog Number:

  E1A0471A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CREB around the phosphorylation site of serine121 (T-D-SP-Q-K).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P16220

• Applications:

  WB: 1:500~1:1000 ELISA: 1:5000

• Other Names:

  CREB-1, CREB1, cAMP-response element binding protein

• Specificity Sensitivity:

  CREB (Phospho-Ser121) antibody detects endogenous levels of CREB only when phosphorylated at serine 121.

• References:

  Lionel Groussin, J. Clin. Endocrinol. Metab., Jan 2000; 85: 345 - 354. Deepa B. Shankar, Blood (ASH Annual Meeting Abstracts), Nov 2005; 106: 367. LA Berkowitz and MZ Gilman, PNAS, Jul 1990; 87: 5258. Michelle L. Kingsley-Kallesen, J. Biol. Chem., Nov 1999; 274: 34020.

• All Sites:

  Human: Ser121; Mouse: Ser121; Rat: Ser121

Western blot analysis of extracts from HeLa cells, treated with Etoposide (25uM, 24hours), using CREB (Phospho-Ser121) antibody.