• Catalog Number:

  E1A0469A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CDK1/CDC2 around the phosphorylation site of threonine 14 (E-G-TP-Y-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P24941

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  CDC28, CDC2A, CDK1, Cell division control protein 2 homolog, Cyclin-dependent kinase 1, EC 2.7.11.22, EC 2.7.11.23, MPF, kinase Cdc2, p34 protein kinase

• Specificity Sensitivity:

  CDK1/CDC2 (Phospho-Thr14) antibody detects endogenous levels of CDK1/CDC2 only when phosphorylated at threonine 14.

• References:

  Carsten Müller-Tidow, Mol. Cell. Biol., Oct 2004; 24: 8917 - 8928. Yukinobu Arata,J. Biol. Chem., Feb 2000; 275: 6337. Patrick Corsino, Cancer Res., Apr 2007; 67: 3135 - 3144.

• All Sites:

  Human: Thr14; Mouse: Thr14; Rat: Thr14

P-peptide - + Western blot analysis of extracts from HepG2 cells, treated with Forskolin (40nM, 30mins), using CDK1/CDC2 (Phospho-Thr14) antibody. Immunofluorescence analysis of COS7 cells, using CDK1/CDC2 (Phospho-Thr14) antibody.