• Catalog Number:

  E1A0468A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CD3 ζ around the phosphorylation site of tyrosine 142 (G-L-YP-Q-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P20963

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  CD3-zeta, T-cell receptor T3 zeta chain, T-cell surface glycoprotein CD3 zeta chain precursor, T3Z, TCRZ

• Specificity Sensitivity:

  CD3 ζ (Phospho-Tyr142) antibody detects endogenous levels of CD3 ζ only when phosphorylated at tyrosine 142.

• References:

  Linda A. Trimble, Blood, Aug 2000; 96: 1021 - 1029. Sandeep Krishnan, J. Immunol., Sep 2005; 175: 3417 - 3423. Linda A. Trimble, J. Virol., Aug 2000; 74: 7320 - 7330. Hisashi Arase, J. Immunol., Jan 2001; 166: 21.

• All Sites:

  Human: Tyr142; Mouse: Tyr143

Western blot analysis of extracts from Jurkat cells, treated with UV (15mins), using CD3 ζ (Phospho-Tyr142) antibody.