• Catalog Number:

  E1A0466A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CBL around the phosphorylation site of tyrosine 700 (T-E-YP-M-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P22681

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  CBL E3 ubiquitin protein ligase, CBL2, EC 6.3.2.-, Proto-oncogene c-CBL, Signal transduction protein CBL

• Specificity Sensitivity:

  CBL (Phospho-Tyr700) antibody detects endogenous levels of CBL only when phosphorylated at tyrosine 700.

• References:

  Joazeiro C.A., Science 286:309-312(1999). Brdicka T., J. Exp. Med. 196:1617-1626(2002). Salomon A.R., Proc. Natl. Acad. Sci. U.S.A. 100:443-448(2003).

• All Sites:

  Human: Tyr700; Mouse: Tyr698; Rat: Tyr806

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using CBL (Phospho-Tyr700) antibody. Western blot analysis of extracts from K562 cells, treated with Na3VO4 (0.3nM), using CBL (Phospho-Tyr700) antibody.

P-peptide - + Immunofluorescence analysis of HepG2 cells, using CBL (Phospho-Tyr700) antibody.