• Catalog Number:

  E1A0463A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Calnexin around the phosphorylation site of serine 583 (N-R-SP-P-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P27824

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  CALX, CANX, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90

• Specificity Sensitivity:

  Calnexin (Phospho-Ser583) antibody detects endogenous levels of Calnexin only when phosphorylated at serine 583.

• References:

  Shaun P Brothers, J. Mol. Endocrinol., Dec 2006; 37: 479 - 488. Vilasack Thammavongsa, J. Biol. Chem., Sep 2005; 280: 33497 - 33505. Yasushi Okazaki, J. Biol. Chem., Nov 2000; 275: 35751. Milka Popov, J. Biol. Chem., Jun 1999; 274: 17635.

• All Sites:

  Human: Ser583; Mouse: Ser582; Rat: Ser582

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Calnexin (Phospho-Ser583) antibody. Western blot analysis of extracts from HeLa cells, treated with EGF (200ng/ml, 30mins), using Calnexin (Phospho-Ser583) antibody.

P-peptide - + Immunofluorescence analysis of HeLa cells, using Calnexin (Phospho-Ser583) antibody.