• Catalog Number:

  E1A0462A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Caldesmon around the phosphorylation site of serine 789 (V-T-SP-P-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q05682

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  CAD, CALD1, CDM, L-caldesmon, Non-muscle caldesmon

• Specificity Sensitivity:

  Caldesmon (Phospho-Ser789) antibody detects endogenous levels of Caldesmon only when phosphorylated at serine 789.

• References:

  Robert Eves, J. Cell Sci., May 2006; 119: 1691 - 1702. Shigeko Yamashiro, Mol. Biol. Cell, Jan 2001; 12: 239. KS Warren, J. Cell Biol., Apr 1994; 125: 359. Ping-Pin Zheng, Am. J. Pathol., Jun 2004; 164: 2217 - 2228.

• All Sites:

  Human: Ser789; Mouse: Ser560; Rat: Ser534

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Caldesmon (Phospho-Ser789) antibody. Western blot analysis of extracts from HeLa cells, treated with EGF (200ng/ml, 30mins), using Caldesmon (Phospho-Ser789) antibody.

P-peptide - + Immunofluorescence analysis of HeLa cells, using Caldesmon (Phospho-Ser789) antibody.