Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human BID around the phosphorylation site of serine 78 (S-E-SP-Q-E).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse
SwissProtNo:
P55957
Applications:
IHC: 1:50~1:100 ELISA: 1:5000
Other Names:
BH3 interacting domain death agonist
Specificity Sensitivity:
BID (Phospho-Ser78) antibody detects endogenous levels of BID only when phosphorylated at serine 78.
References:
Peter Bogetoft. Eur. Rev. Agric. Econ., Jun 2003; 30: 193 - 215.
Sanjay Pradhan. J. Immunol., Nov 2006; 177: 5956 - 5967.
Takuya Fukazawa. J. Biol. Chem., Jul 2003; 278: 25428 - 25434.
Anthony J. Valentijn. J. Biol. Chem., Jul 2004; 279: 32848 - 32857.
All Sites:
Human: Ser78; Mouse: Ser78
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human brain tissue using BID (Phospho-Ser78) antibody.