• Catalog Number:

  E1A0459A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Aurora Kinase around the phosphorylation site of threonine 288 (R-T-TP-L-C).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O14965

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  AIK, ARK1, AYK1, Aurora-A, Aurora-family kinase 1, Aurora-related kinase 1, Aurora/IPL1-related kinase 1, BTAK, Breast-tumor-amplified kinase, EC 2.7.11.1, IAK1, Ipl1- and aurora-related kinase 1, STK15, STK6, Serine/threonine kinase 15, Serine/threonine kinase 6, Serine/threonine kinase Ayk1, kinase AurA

• Specificity Sensitivity:

  Aurora Kinase (Phospho-Thr288) antibody detects endogenous levels of Aurora Kinase only when phosphorylated at threonine 288.

• References:

  Amy E. Geddis, Blood, Aug 2004; 104: 1017 - 1024. Edmund Chun Yu Lee, Cancer Res., May 2006; 66: 4996 - 5002. Kaori Sasai, AACR Meeting Abstracts, Apr 2005; 2005: 608. T Marumoto, Genes Cells, Nov 2002; 7: 1173 - 1182.

• All Sites:

  Human: Thr288; Mouse: Thr279; Rat: Thr281

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Aurora Kinase (Phospho-Thr288) antibody. Western blot analysis of extracts from 293 cells, treated with serum (20%, 15mins), using Aurora Kinase (Phospho-Thr288) antibody.