Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human AS160 around the phosphorylation site of threonine 642 (A-H-TP-F-S).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse
SwissProtNo:
O60343
Applications:
IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000
Other Names:
Akt substrate of 160 kDa, KIAA0603, TBC1 domain family member 4, TBC4, TBCD4
Specificity Sensitivity:
AS160 (Phospho-Thr642) antibody detects endogenous levels of AS160 only when phosphorylated at threonine 642.
References:
Eric B. Taylor, FASEB J, Apr 2007; 21: A1207.
Henning F. Kramer, J. Biol. Chem., Oct 2006; 281: 31478 - 31485.
Grantley R. Peck, Mol. Endocrinol., Oct 2006; 20: 2576 - 2583.
All Sites:
Human: Thr642; Mouse: Thr649
P-peptide - +
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using AS160 (Phospho-Thr642) antibody.
Immunofluorescence analysis of HeLa cells, using AS160 (Phospho-Thr642) antibody.