• Catalog Number:

  E1A0453A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human AKT1S1 around the phosphorylation site of threonine 246 (L-N-TP-S-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q96B36

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

  Akt1 substrate 1 (proline-rich), Lobe, MGC2865, PRAS40, proline-rich Akt substrate 40 kDa

• Specificity Sensitivity:

  Akt1 S1 (Phospho-Thr246) antibody detects endogenous levels of AKT1S1 only when phosphorylated at threonine 246.

• References:

  Mayumi Yoshioka, J. Mol. Endocrinol., Aug 2007; 39: 119 - 133. Bruno D. Fonseca, J. Biol. Chem., Aug 2007; 282: 24514 - 24524. Kerstin Stemmer, Cancer Res., May 2007; 67: 4052 - 4068. Linglin Xie, J. Biol. Chem., Jul 2007; 10.1074/jbc.M604857200.

• All Sites:

  Human: Thr246; Mouse: Thr247; Rat: Thr262

Western blot analysis of extracts from HepG2 cells, treated with PDGF (50ng/ml, 30mins), using Akt1 S1 (Phospho-Thr246) antibody.