• Catalog Number:

  E1A0451A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ADD1 around the phosphorylation site of threonine 445 (E-K-TP-R-W).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P35611

• Applications:

  IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  ADDA, Alpha adducin, Erythrocyte adducin alpha subunit, adducin, alpha

• Specificity Sensitivity:

  ADD1 (Phospho-Thr445) antibody detects endogenous levels of ADD1 only when phosphorylated at threonine 445.

• References:

  Jae Bum Kim, PNAS, Apr 1998; 95: 4333. Kang Ho Kim, J. Biol. Chem., Dec 2004; 279: 51999 - 52006. Marc Foretz, Mol. Cell. Biol., May 1999; 19: 3760 - 3768 David E. Tabor, J. Biol. Chem., Jul 1999; 274: 20603.

• All Sites:

  Human: Thr445; Mouse: Thr445; Rat: Thr445

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ADD1 (Phospho-Thr445) antibody.